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The membrane pellet was homogenized and solubilized by gentle nutation for 2 hours at 4 °C in buffer consisting of 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, 1% Dodecyl-β-D-Maltosid (w/v, DDM), and 20 µM DNQX, followed by centrifugation at 200,000 g for 30 min to remove insoluble material

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10.1155/2019/9694212 [DOI] [PMC free article] [PubMed] [Google Scholar] 312.Herrera-Arellano A, Miranda-Sánchez J, Avila-Castro P, Herrera-Alvarez S, Jiménez-Ferrer JE, Zamilpa A, et al