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For MST analysis, αSyn variants, αSyn WT -141C, αSyn S129E -141C, and αSyn 1–103 -104C with a single cysteine residue near the C-terminus end were labeled using the Monolith NT™ Protein labeling Kit RED-MALEIMIDE (NanoTemper Technologies)

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Importantly, we highlighted that astrocytes may participate in these processes by enhancing glutamate secretion and its extracellular levels (producers) and abnormally responding to the augmented glutamate concentration (targets)