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In the recent study, the experimenters first cultured primary cortical neurons pretreated with METH and then subjected them to high-throughput RNA sequencing to screen for circRNA-specific expression

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In the cultures treated with B12, 45 and 74 out of these 90 pathways were still differentially regulated in MOD and SEV groups, respectively, although with a minimum 20% difference in Z-scores relatively to their untreated condition