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The resulting V H and V L gene fragments were cloned into the multicloning site of the appropriate pTT5 heavy or light chain vector (either kappa or lambda light chain), as described above
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This is particularly relevant for complex matrices, and today mass spectrometry analysis of the 4-HNE adductome by liquid chromatography tandem mass spectrometry (LC-MS/MS) is an indispensable tool
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