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The FTIR spectra of empty MNP, T0-CRISPR/Cas9-PEI-MNPs, and T3-CRISPR/Cas9-PEI-MNPs exhibit a broad absorption peak from 2972 to 3339 cm −1 , which is due to the stretching vibration of the −OH group arising from the hydroxyl group of nanoparticles, DNA and water molecule 33,34

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