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Cells were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO 2
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In some cancer cells, mitochondrial ETC activity promotes ferroptosis, at least in response to cystine deprivation, as shown using specific ETC poisons 89
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