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FACS Analysis Single-cell suspensions from whole thymus, the APC-enriched fraction after Percoll, DC and DC-depleted fractions were stained with antibodies against HLA–DR (PerCP, 307628, 1:50), CD19 (FITC, 302205, 1:20), CD14 (APC, 301807, 1:50), CD45RA (FITC, 304105, 1:10), CD123 (PE/Cy7, 306009, 1:10), (all from Biolegend), CD45 (PB, PB986, 1:10, DAKO), BDCA-4 (PE, 130-090-533, 1:10), CD11c (PE, 130-092-411, 1:10), EpCAM (APC, 130-091-254, 1:10, from Miltenyi Biotec), CDR2 (1:50, Bruno Kyewski, DKFZ, Heidelberg
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