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Size:1000 mcg/ml - 30 ml x 4
Then, the cells were treated consecutively with oligomycin (an ATP synthase inhibitor, 1.5 μM), FCCP (carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone, an uncoupling agent, 2 μM), and antimycin A and rotenone (complex III and I inhibitors, respectively, 0.5 μM) every 20 min
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