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The plate was placed into a 37 °C incubator for 2 hours before 2 µL of a 5 µM dBET6 solution in RPMI with 10% FBS was added to each well, and the plate was again placed on an orbital shaker for 15 seconds (final concentration of dBET6 is 500 nM, control wells are treated with an equal volume of DMSO in RPMI with 10% FBS)

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Many patients also consider DSIP when they wake up too early, feel groggy in the morning, or feel like sleep is “light” and not refreshing