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SAM donates its methyl group, generating S-adenosyl homocysteine, which is then hydrolyzed to adenosine and homocysteine

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Furthermore, alanine mutations of the established glutathione-scaffold site residue D90 or of crucial residue K26 in monothiol PfGrx C32S/C88S slowed down the reaction and resulted in linear secondary diagrams for the first rate constant (Fig