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The NAM resulting from NAD + consumption subsequently re-enters the salvage pathway and thus the NAD + synthesis cycle (Fig

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This article systematically reviews the core strain characteristics, antitumor molecular mechanisms, genetic engineering and synthetic biology modification strategies of oncolytic bacteria, summarizes preclinical research results and early clinical trial progress, deeply analyzes the core challenges currently faced in clinical translation, such as biosafety, delivery efficiency, and efficacy evaluation, and looks forward to future development directions combined with multidisciplinary cross-technologies, aiming to provide comprehensive theoretical support and practical references for deepening basic research and clinical translation of oncolytic bacteria in the field of malignant glioma

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