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Further studies with larger cohorts, multiple dosing regimens, extended reperfusion periods, longer follow-up, and more comprehensive mechanistic investigations are required to validate and extend these findings

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In addition, acrolein exerts its biological effects through reaction with nucleophilic sites in proteins, i.e ., the sulfhydryl group of cysteine, the imidazole moiety of histidine, and the ε-amino group of lysine, thereby impairing protein function