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Total Activity also goes down with each purification step, as some of your protein of interest is also lost at each purification step because (1) some protein will stick to the test tubes and glassware, (2) some protein won't bind with 100% efficiency to your column matrix, (3) some protein may bind too tightly to be removed from the column matrix during elution, and (4) some protein may be denatured or degraded during the purification process
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