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The l-EV or s-EV fractions prepared from FBS (6 × 10 8 particles) were added to the confluent monolayer of PANC-1 cells in 1 ml of DMEM medium containing 10% (vol/vol) of EV-depleted FBS in a 35 mm dish (Corning, #430165) after scratch
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They reported that p53 suppresses accumulation of cytoplasmic chromatin fragments (CCF), including DNA damage signaling marker γH2A.X, and its downstream inflammatory phenotype (e.g., SASP) of monocytes and macrophages in liver
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