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After cooling, 10 µl from each sample was loaded on Bis-Tris 4-20% gradient gels (GenScript) and run at 55 mAmp per gel until the colour ran out
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Previous study has shown that IL-13 produced by Th2 cells is instrumental in driving fibroblast activation and ECM production in fibrotic tissues, underscoring its significance in the fibrotic process (107)
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