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It was further shown that NPS applied intracerebroventricularly was able to reduce airway responsiveness to methacholine in experimental animals, most likely through a CNS-mediated pathway connecting respiratory and stress responses [35]

Peptide Complex

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The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA Total Protein Assay (Pierce), and then appropriate volumes of the lysates were diluted with 2× reducing Laemmli buffer and heated for 10 minutes at 95°C