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Ions, such as Na + , K + , Fe 2+ /Fe 3+ , Mg 2+ , Ca 2+ , Cl − , HPO 4 2- /H 2 PO 4 − , and HCO 3 − in FBS help to regulate osmotic balance and homeostasis in culture media, reducing cellular damage from shifts in osmotic pressure and pH (Lee D
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However, this practice inevitably narrows the clinical spectrum represented in research samples and reduces natural heterogeneity of ASD, limiting the generalizability of findings
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