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ABCC1 was eluted with 300 mM NaCl, 50 mM Tris-HCl pH 8.0, 400 mM imidazole, 0.02% (w/v) GDN and 5% glycerin, supplemented with 2 mM DTT

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Typical regimens include: A high-potency multivitamin Calcium citrate (often 1,500–2,000 mg per day in divided doses) Vitamin D, often at higher doses than the standard population Iron, especially in menstruating women Vitamin B12, by tablet, sublingual, or injection Fat-soluble vitamins A, E, and K, often as a combined preparation Specific doses are adjusted based on blood-test results and individual needs