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Flow cytometry on non-permeabilized cells is the correct method β a western blot band from the total lysate says nothing about membrane localization
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For instance, ROS can directly oxidize and damage pancreatic Ξ²βcells, induce Ξ²βcell apoptosis, and indirectly inhibit Ξ²βcell function by affecting insulin signalling pathways, thereby causing fluctuations in blood sugar levels
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