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In summary, the NF-κB signaling pathway is critical in the process of GABA exerting its role in regulating neuroinflammation on macrophages, astrocytes and microglia, and may be a key therapeutic target for inhibiting neuroinflammation and improving insomnia diseases

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Collectively, our results provide compelling evidence that dual antagonists targeting mGluR5 and 5-HT2aR can effectively reduce allodynia and pain intensity, potentially through the modulation of downstream signaling molecules such as p-ERK1/2 and p-AKT