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The digested plasmid was gel-purified using NucleoSpin Gel and PCR Clean-Up (Macherey-Nagel) and ligated overnight at 16 °C using T4 DNA ligase (NEB) with a codon-optimized sequence of GLS
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or (3) electron bifurcation mechanisms by which two electrons bifurcate to a high and low potential pathway (Ref
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