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Estimation of total free amino acids, proline and proline metabolism enzymes The Yokoyama and Hiramatsu’s 87 ninhydrin method, with some modifications was used to measure total free amino acids in root extracts at 570 nm using 80% ethanol as blank against l-leucine (Sigma, cat
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In the ‘down’ state, RBD angles are close to the central cavity of the trimer to shield the receptor-binding regions, while in the ‘up’ state, the RBD undergoes hinge-like conformational movement, exposing its determinant regions to recognize the human angiotensin-converting enzyme 2 (hACE2) receptor on the host cellular membrane, the state of which is considered to be less stable than in the ‘down’ state
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