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A funnel plot is suggestive of the presence of most of the studies outside the plot, hence it is likely that significant publication bias is present DISCUSSION Obesity has severe health consequences, with each 5 kg/m 2 increase in BMI beyond the normal range linked to a 30% increase in mortality and a potential reduction in life expectancy by up to 10 years.[16] Conversely, significant weight loss is associated with metabolic and non-metabolic benefits

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Immunofluorescence staining was performed to assess macrophage polarization, with CD86 serving as a marker for pro-inflammatory M1 macrophages and CD206 for anti-inflammatory M2 macrophages