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At least 15 independent leaf discs of 1 cm 2 within the infected area in each treatment of one independent experiment were excised and the bacterial populations in the leave discs were determined in KB medium containing 1.5% (m/v) agar and 50 ΞΌg ml β1 rifampicin using 10 ΞΌl five 10-fold dilutions of homogenate of Pst DC3000-infected leaves as described in Shi et al
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