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The RNP solution (1 µl) was electroporated with 200,000 cells in electroporation buffer and the cells were deposited into antibiotic-free medium, as described above, and allowed to rest in a 37 °C incubator for 24 h before additional antibiotic-containing medium was added

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For SDS–PAGE, 80 μL of each fraction were mixed with 40 μL of 2× sample buffer (62.5 mM Tris-HCl, pH 6.0, 8 M urea, 2% SDS, 4% β-mercaptoethanol), heated at 90°C for 2 min, and 25 μL (corresponding to ∼3% of each fraction) were resolved on 8% polyacrylamide gels