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Consistent with these data, single-cell RNA-Seq (scRNA-Seq) analysis showed that DR decreased the expression of NADK in several cell types in brown adipose tissue (BAT) and bone marrow, while DR also decreased the expression of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of NAD + salvage synthesis, and PPP enzyme transoldolase (TALDO) in a cell population in WAT, which may lead to decreased NADP(H) levels and decreased NADPH oxidase activity, resulting in decreased inflammation
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When injections finished, all mice were euthanized, and the serum was harvested
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