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This medication induces protective autophagy by activating the AMPK signaling pathway, effectively inhibiting apoptosis and senescence in NP cells
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The following day, the cultures were diluted 1:100 into 1L LB broth and allowed to grow at 37°C while shaking at 280 rpm until the cultures reached an optical density at 600 nm (OD 600 ) of 0.6, measured by NanoDrop One (Thermo Fisher Scientific, Cat# 13–400-518)
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