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Samples (20 µl) were mixed with 5 µl of 5x loading buffer (0.3 M Tris-HCl pH = 6.8, 25% β-mercaptoethanol, 50% glycerol, 10% SDS, 1% bromophenol blue), heated to 95 °C for 5 min, and 15 µl of samples were applied to the gel together with 5 µl of “Thermo Scientific PageRuler Unstained Broad Range Protein Ladder”
skin barrier
Type:69g
A dose-dependent decrease in body weight was shown, ranging from -1.3 kg in the placebo group to -1.4 kg in the 0.03 mg/kg leptin-treated group, and to -7.1 kg in the 0.30 mg/kg leptin-treated group (8)
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