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Materials & methods Cell culture Caco-2 cells (passages 10–18) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and HT29-MTX cells (passage 8–16) were obtained from the Max Rubner-Institut (MRI, Karlsruhe, Germany)

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The supernatant was incubated overnight with 3 μg of the appropriate antibody with rotation and precipitated by the addition of 30 μl of protein-A/G beads at 4 °C and mixing for an additional 3 h