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Then transfer the survival cells into a 96-well plate to expand single clones for approximately 3 weeks, genomic DNA were extracted and sequencing after PCR amplification using the following primers TLK1: F: TCACTTTGTCAGCGTGGTCA, R: CCACGGTCTGACTGCAAAAA
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In addition, four ferroptosis-related genes: NOX4, MTCH1, GABARAPL2, and SLC2A3, were identified and shown to accurately predict GC and H
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