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Specimens were subsequently dehydrated in a series of ethanol washes (50% to 100%), critical-point-dried with carbon dioxide (Samdri-790, Tousimis, Rockville, USA), mounted on a carbon tape with an aluminum stud, and coated with 20 nm gold in a EM ACE600 Sputter coater (Leica, Germany)
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Clinical Research and Public HealthNeuroscienceOphthalmology Open Access | 10.1172/jci.insight.145346 1 Institute of Metabolism and Systems Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
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