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qPCR was performed with SYBR Green Supermix (TaKaRa) with the protocol as follow: 95 °C for 5 min, repeating 40 cycles of 95 °C for 5 s and 60 °C for 45 s using StepOnePlus real-time PCR system (Applied Biosystem, Foster City, CA, USA)

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Oral supplementation is appropriate for patients with dietary insufficiency and intact absorption mechanisms