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In the case of hypoxia/reoxygenation (H/R)-induced injury, senescent endothelial cells have a higher rate of apoptosis, excessive ROS, insufficient nitric oxide (NO), upregulated nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2), downregulate angiotensin-converting enzyme 2 (ACE2) and eNOS, and compromised tube formation ability

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However, glutathione production declines with age , and factors like poor diet, high stress, alcohol consumption, and exposure to toxins further deplete levels