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BAC Water | 0.9% Benzyl Alcohol, 30 mL – C2 Peptides https://www.jove.com/files/ftp_upload/17180/17180_article_thumb_17180.jpg Size:100 Grams Compounding Optimal storage conditions: | Condition | Recommendation | |---|---| | Temperature (routine) | -20°C | | Temperature (long-term) | -80°C | | Atmosphere | Desiccated | | Light | Protected from direct light | | Humidity | 5% and is difficult to remove downstream **Methanol or ethanol** — can cause peptide precipitation when diluted into aqueous buffers **Strong acids or bases** — can hydrolyse peptide bonds **Buffers with primary amines** (Tris, glycine) — can react with peptide N-termini or lysine side chains Reconstitution Step-by-Step A standard reconstitution protocol: Equilibrate the lyophilised vial to room temperature (15-30 minutes) Calculate the required solvent volume: **volume (mL) = (vial mass mg × net peptide content %) / target concentration (mg/mL)** Draw the calculated solvent volume into a sterile syringe Insert the needle into the vial at an angle, directing the solvent stream against the inside wall of the vial (NOT directly onto the lyophilised powder) Roll the vial gently between palms or use a low-speed orbital mixer until the powder is fully dissolved Visually verify the solution is clear and free of particulates before use If the powder does not dissolve within 2-3 minutes of gentle swirling, the solvent choice may be wrong